Microbiome

Microbiome



The microbiome consists of microbes that are both helpful and potentially harmful. Most are symbiotic (where both the human body and microbiota benefit) and some, in smaller numbers, are pathogenic (promoting disease). In a healthy body, pathogenic and symbiotic microbiota coexist without problems. But if there is a disturbance in that balance—brought on by infectious illnesses, certain diets, or the prolonged use of antibiotics or other bacteria-destroying medications—dysbiosis occurs, stopping these normal interactions. As a result, the body may become more susceptible to disease.

https://www.hsph.harvard.edu/nutritionsource/microbiome/ 


Current understanding of the human microbiome

 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7043356/

Human Microbiome Project:

If humans are thought of as a composite of microbial and human cells, the human genetic landscape as an aggregate of the genes in the human genome and the microbiome, and human metabolic features as a blend of human and microbial traits, then the picture that emerges is one of a human 'supra-organism'.

The microorganisms that live inside and on humans (known as the microbiota) are estimated to outnumber human somatic and germ cells by a factor of ten.

If humans are thought of as a composite of microbial and human cells, the human genetic landscape as an aggregate of the genes in the human genome and the microbiome, and human metabolic features as a blend of human and microbial traits, then the picture that emerges is one of a human 'supra-organism'.

it is hoped that the HMP will not only identify new ways to determine health and predisposition to diseases but also define the parameters needed to design, implement and monitor strategies for intentionally manipulating the human microbiota, to optimize its performance in the context of an individual's physiology.


https://www.nature.com/articles/nature06244 



Gut Brain Axis

The gut microbiota is the set of microorganisms present in the gut, and it is connected to the central nervous system via the gut-brain axis

numerous studies have demonstrated its involvement in human behaviour and its relationship with several pathologies

The results showed that gut dysbiosis predicts the development of these pathologies and influences their pathogenesis

it was found that different psychobiotics, mainly dietary fibers and probiotics of the Lactobacillus family, improved different cognitive functions such as cognitive performance and induce a reduced cortisol response
The gut microbiota-brain axis, psychobiotics and its influence on brain and behaviour: A systematic review
https://doi.org/10.1016/j.psyneuen.2021.105640


Psychobiotics are probiotics strains with potential benefits for host physical health and is widely used for mental disease therapy.

The gut-brain axis is bidirectional association between the gastrointestinal system and the CNS that coordinates the functions of the ENS and CNS

The  term  “Psychobiotics”  refers  to  probiotics,  prebiotics,  and all microbiota-targeted interventions that can manipu-late microbiota-gut-brain signals and have positive effects on neurological functions such as mood, cognition, and anxiety

Fermented foods including yogurt, kefir, tempeh, and kimchi are rich sources of probiotics


GABA  is  an  amino  acid  with  a  non-protein  conformation and one of the secretory metabolites of pro-biotics, especially lactic acid bacteria (LAB)

Remarkably, the food industry has a critical role in reducing depression and anxiety in the society by producing functional foods con-taining probiotic species with the ability to produce GABA

It is defined that Psychobiotics are a subclass of probiotics that bring mental health benefits to the host by interacting with gut bacteria as well as oncobiotics, pharmabiotics, and metabiotics

In addition to their positive psychological effects, Psycho-biotics could induce the production of neuro-transmitters and neuro-hormones that exhibit psychotropic effects in the studied models.

confirmed that kefir grains contain species such as Acetobacter aceti, L. fructivorans, Acetobacter sp., Enterococcus faecium, Leu-conostoc spp., L. delbrueckii delbrueckii, L. fermentum, L. kefiranofaciens, Candida famata, and Candida krusei
|Note|Water Kefir and Milk Kefir

they reported positive effects of these strains in improving memory, visual-spatial and abstraction proper-ties, as well as executive and language functions in Alz-heimer
|Note|Kefir Grains 

Likewise, the probiotic strain L. helveticus  was  isolated  from  fermented  milk  which  can  improve cognitive function in elderly and middle-aged adults

Saccharomyces cerevisiae

that fermented milk with L. casei Shirota can improve mood, increase fecal serotonin, and reduce stress level in the investigated students
Psychobiotics: the Influence of Gut Microbiota on the Gut-Brain Axis in Neurological Disorders - s12031-022-02053-3.pdf
https://link.springer.com/content/pdf/10.1007/s12031-022-02053-3.pdf




Cogito Ergo Sum Bacterium

Are we humans really the individual, bounded selves we take ourselves to be? 
Microbiome science is also confounding a long tradition in anatomy and physiology that defines our individual identity in terms of the higher functions of the human brain mediating self-awareness, personality traits, and emotional state

In addition, short chain fatty acids, which are metabolic waste products of gut microorganisms, promote the differentiation of B cells into plasma cells that secrete protective immunoglobulin A molecules (IgAs) [12], and there is evidence that the gut microbiome has an important systemic effect of inhibiting the IgEs that mediate many allergic diseases (asthma, eczema, etc.), severe systemic reactions to allergens, and some autoimmune diseases

Therefore, the experimental evidence for microbiome effects on behavioral traits that we consider to define our sense of self––who and what we are––has profound implications beyond their biomedical significance and especially for our philosophical comprehension of the human self

his fluidity in host–microbial relations has two profound consequences. The first––specific––one is that the scope of genome-based precision medicine [27] needs to be reassessed in the light of the evidence that many medically important traits are not shaped exclusively by the human genetic makeup but depend to a significant degree on the genetic capabilities of the microbiome [28]. The second––more far-reaching––consequence is that the growing realization that much of the genetic constitution of every human body is microbial [8] radically undermines any definition of “self” in terms of our individual human genome

We want to stress that the discovery of the foundational importance of the microbiome for the genetic constitution of the human is qualitatively different from the much older argument that the environment has an influence over our genome. It is qualitatively different, first, insofar as the older distinction left untouched the assumption that it is our nuclear genome that is alone constitutive of the human individual self and merely granted the environment some “influence” over the genome. It is qualitatively different, second, because what is at stake is not actually influence: The microbiome is not “influencing” the genome; it is coconstituting the metaorganisms we humans are.

growing realization that the composition and activities of our microbial partners are directly involved in the key biological processes that define traditional concepts of the self

Textbooks are being revised and lecture courses redrafted to accommodate the new biology of the microbiome.

the finding that microorganisms are a constitutive part of ourselves calls for a new configuration of the effort to understand what it means to be human

As we see it, it is important but not enough to argue that “we have never been individuals” [3]––or to suggest that human and microbial worlds are inseparably “entangled” [30–32].

What is needed, in addition, is a whole new configuration of research, one where arts and science are combined.

what it means to be a living human being at home in a microbial world, one on which we depend and with which we are inseparably interwoven

after the illusion of the bounded, individual self. The human is more than the human


How the microbiome challenges our concept of self

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5823462/



The key question about biological individuality raised by microbiome research is the extent to which our identity as an evolutionary or physiological individual depends on the microbes living in and on u

We agree that causal relationships between individual microbes or small groups of microbes and the host are very likely to impact how the host survives and thrives in daily life.

Many microbiome participants are known empirically and theoretically to be transient, opportunistic, or even inactive

For instance, we might establish the “identity” of a criminal through genetic material left at the scene and then excuse the crime on the basis of the criminal’s state of mind during the crime (she was not “herself”).

We are challenging the authors’ suggestion that the humanities and arts need to be fundamentally overhauled in light of microbiome research (their call for a “whole new configuration of research” resulting in “microbial humanities”).

We wholeheartedly agree with Rees and colleagues that the humanities and the natural sciences should not operate in isolation from one another
A cautionary note for claims about the microbiome’s impact on the “self” - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135347/


Holobionts

The Microbiome is in part responsible for mental and physical health - 
Investigating methods for treating the entire microbiome for the symptoms of Crohns.




Three  claims  might  be  taken  as  implicit  in  this  language:

that  many  of  themicrobial  and  macrobial  taxa  associated  in  such  multi-species  assemblages  aremetabolically  (or  developmentally  or  structurally)  interdependent

that  suchinterdependence has co-evolved as a consequence of their association, and

thatholobionts  can  be  considered  units  of  selection

Making the case that interaction patternsfulfill  the  criteria  associated  with  units  of  selection  does  require  a  relativelyW. F. Doolittle, A. Booth123
substantial rethinking of various evolutionarily relevant concepts, especially that of‘‘reproduction.’

we  have  known  for  many  decades  that  major  biogeochemicalcycles  in  the  ocean  or  on  land  are  driven  by  microbes  and  that  microbes  affectanimal,  human, and  plant  health directly  in many  ways,  not all  negative,

Advances  will  come  from  empirical  studies  thatstart   with   rigorous   assumptions   and   a   clear   framework   for   detectingcoevolution  and  teasing  apart  levels  of  selection

the  microbial  taxa  that  partner  with  any  given  hostmacrobe  or  with  each  other  can  be  highly  variable  taxonomically—not  the  sameIt’s the song, not the singer: an exploration of...123
strains,  the  same  species,  or  even,  sometimes,  the  same  phyla.  Many  holobiontsnevertheless maintain relative functional stability by recruitment of microbial taxa,physiologically or biochemically equivalent but not necessarily of the same geneticlineages, from the environment

These authors suggest that an appropriately ecological microbial classification wouldrecognize  functional  ‘‘guilds,’’  phylogenetically  heterogeneous  groups  defined  onlyby  their  possession  of  one  or  more  biochemical  capacities

It's the song, not the singer: an exploration of holobiosis and evolutionary theory - Doolittle_Booth_holobiont_evolution_2016.pdf
https://www.zoology.ubc.ca/bdg/pdfs_bdg/2016 Fall/Doolittle_Booth_holobiont_evolution_2016.pdf


And functional gastrointestinal disorders such as irritable bowel syndrome represent a disorder of the axis, rather than an isolated problem either of psychology or of gastrointestinal function

We are living in a microbial world

|Note|MAterial World MAdonna

the brain–gut–microbiome axis has become a major research area at the interface of neuroscience and microbiology

he study of how microbes within the body can interact with human brain and behaviour can offer a more complete understanding of human psychology

Freud and others within the psychoanalytical school of thought placed a great emphasis on unconscious processes in human psychology

|Note|the microbes made me do it

the brain–gut–microbiota axis represents a further, physiological means through which unconscious processes may impact on our behaviour

but also leads to a subjective appraisal that is driven by an emotional response that is in turn tied up with a bodily response to the information

there has been little research integrating the study of the mind and brain with the microbiological study of the bacteria in the human body, especially the gut

have described it as nothing less than a paradigm shift in neuroscience; this is clearly an area in which the discipline of psychology could make a substantial contribution

highlighting the potential role of this axis in interpersonal social psychology

The gastrointestinal tract also contains an enteric nervous system comprising an interconnected network of neurons, similar in number to the neurons in the spinal cord (Sasselli, Pachnis, & Burns, 2012).

The brain sends signals to the gut, which impacts upon its sensory and secretory function, and in return receives visceral information from the gut

A great volume of interoceptive information is sent from the gut to the brain, and much of this will not be processed consciously (Mayer, 2011),

|Note|Interoceptive: relating to stimuli produced within an organism, especially in the gut and other internal organs."In our study, we used the five items of the Private Body Consciousness subscale as a measure of interoceptive awareness."

bacteria found within the gut can produce neurotransmitters that can also be found within the central nervous system

For example, evidence suggests that a strain of Lactobacillus brevis can produce GABA

Nonetheless, a propensity to heightened anxiety may also be transmitted biologically, not only through human genes, but also via the microbiota via vaginal delivery

The physiological impact of chronic stress can be contrasted with acute stress.

te stress is associated with release of cortisol via the HPA axis

In contrast, chronic stress is associated with dysregulation of the HPA axis

in irritable bowel syndrome (IBS), a disorder of the brain–gut–microbiome axis, HPA axis activation persists for longer than in healthy controls

It is perhaps not surprising that this question has not been well studied in humans, as researchers lack in vivo methods for studying the composition and function of the gut microbiota; assessment typically requires the collection of stool samples.

The gut microbiota has been shown to interact with host cognition in numerous laboratory animal model studies





A psychology of the human brain–gut–microbiome axis.




How to correct this integral microbiome?